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Updated: Apr 26, 2026

Analysis of Hematopoietic Stem Progenitor Cell Metabolism
Published on: November 9, 2019
Metabolic characterization of hematopoietic stem cells
Fatih Kocabas1, Junke Zheng, Chengcheng Zhang
1Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, 3203 N. Sam Houston Pkwy W, Houston, TX, 77038, USA, fk@na.edu.
Abstract:
An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.
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