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A novel germ-line JK transcript starting immediately upstream of JK1
L Leclercq1, P Butkeraitis, M Reth
1Institute of Genetics, University of Cologne, Köln, FRG.
Nucleic Acids Research
|September 12, 1989
Abstract:
Germ-line transcripts of the immunoglobulin (Ig) and T cell receptor loci are thought to be involved in the control of V gene rearrangement by rendering these loci accessible to the recombinases. We have analyzed the transcriptional activity of germ-line K alleles in two bone marrow-derived Abelson-murine leukemia virus transformed pre-B cells: 300-19, a null cell line, and P8 a mu-producing line. We found a novel germ-line JK transcript starting immediately upstream of JK1 and spliced to CK. The potential role of this transcript in the opening of the Ig K locus as well as in the ordered usage of JK segments is discussed.