Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration01:25

Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration

374
Hemoperfusion and hemofiltration are critical techniques in medical treatments to eliminate accumulated drugs, metabolites, and electrolytes from the bloodstream. These methods are particularly vital in cases of accidental poisoning and drug overdose.Hemoperfusion involves passing blood through an adsorbent material to remove unwanted substances. The main adsorbents used in hemoperfusion include activated charcoal and Amberlite resins. Activated charcoal can adsorb both polar and nonpolar...
374
Hemodialysis II: Procedure and Complications01:24

Hemodialysis II: Procedure and Complications

2.1K
DialyzersA hemodialysis (HD) dialyzer is a plastic cartridge containing thousands of parallel hollow fibers, which serve as semipermeable membranes. These fibers are typically made from cellulose-based or other synthetic materials. During HD, blood is pumped into the top of the cartridge and distributed among these fibers. Simultaneously, dialysis fluid, known as dialysate, is introduced into the bottom of the cartridge, bathing the outside of the fibers. Across the semipermeable membrane,...
2.1K
Extracorporeal Removal of Drugs: Continuous Renal Replacement Therapy01:26

Extracorporeal Removal of Drugs: Continuous Renal Replacement Therapy

414
Continuous Renal Replacement Therapy (CRRT) is an essential intervention for patients experiencing severe kidney dysfunction. This therapy offers a continuous mechanism for removing fluids and toxins from the bloodstream, leveraging the patient’s blood pressure to facilitate filtration through a specialized filter. This method contrasts with intermittent dialysis, providing a gentler and more consistent removal of waste products and excess fluid, which is particularly beneficial in...
414
Hemodialysis I: Introduction01:25

Hemodialysis I: Introduction

4.0K
Hemodialysis (HD) is a medical treatment that artificially removes waste products, excess fluids, and toxins from the blood when the kidneys are no longer able to perform these functions effectively. In this process, blood is filtered through a semipermeable membrane, allowing for the selective removal of waste while preserving necessary components like blood cells and proteins. Hemodialysis is typically performed in patients with end-stage renal disease (ESRD) or severe kidney...
4.0K
Extracorporeal Removal of Drugs: Peritoneal Dialysis and Hemodialysis01:30

Extracorporeal Removal of Drugs: Peritoneal Dialysis and Hemodialysis

1.1K
Patients with end-stage renal disease (ESRD) or those experiencing drug overdose often require extracorporeal methods to eliminate accumulated drugs and metabolites. Hemoperfusion, hemofiltration, and dialysis are the primary techniques to rapidly remove harmful substances without disrupting the patient's fluid and electrolyte balance. For those with compromised renal function, dosage adjustments of concurrent medications may be necessary during extracorporeal drug removal.Dialysis is a process...
1.1K
Continuous Renal Replacement Therapy01:30

Continuous Renal Replacement Therapy

2.3K
Continuous Renal Replacement Therapy, also known as CRRT, is a procedural treatment for acute kidney injury (AKI) that gradually removes uremic toxins and fluids while maintaining acid-base balance and stabilizing electrolytes. It is particularly useful for hemodynamically unstable patients. Unlike intermittent hemodialysis, which is faster, CRRT provides a gentler approach over 24 hours, closely mimicking the function of natural kidneys. However, CRRT is not ideal for patients with...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulating Membrane Surface Properties via Prewetting With Polysorbate 20 to Improve Sterile Filtration of Nanoemulsions.

Biotechnology journal·2026
Same author

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP).

Biotechnology progress·2026
Same author

Hydrogenation Kinetics Study: Precise Control of C=C Bonds in Polyisoprene (PI)-Containing Block Copolymers via Diimide Hydrogenation.

ACS applied polymer materials·2026
Same author

Viscosity of concentrated mRNA solutions: Implications for downstream processing.

Biotechnology progress·2026
Same author

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification.

Biotechnology and bioengineering·2026
Same author

Affinity membranes in downstream bioprocessing: From chemical design rules to ligand engineering.

Journal of chromatography. A·2026

Related Experiment Video

Updated: Apr 26, 2026

An Open-Source Normothermic Perfusion System Designed for Research Scientists
11:23

An Open-Source Normothermic Perfusion System Designed for Research Scientists

Published on: July 18, 2025

1.4K

Outside-In Hemofiltration for Prolonged Operation without Clogging.

Stanislav S Dukhin1, Yacoob Tabani1, Richard Lai1

  • 1NovaFlux Technologies, 1 Wall Street, Princeton, NJ 08540, USA.

Journal of Membrane Science
|July 29, 2014
PubMed
Summary

Outside-in hemofiltration (HF) significantly extends filter life beyond 100 hours for renal replacement therapy. This novel approach minimizes thrombosis impact, overcoming limitations of traditional HF methods.

Keywords:
CloggingFilter LifeHemofiltrationOutside-In FiltrationThrombosis

More Related Videos

Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
08:50

Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma

Published on: December 23, 2025

309
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
12:11

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

22.4K

Related Experiment Videos

Last Updated: Apr 26, 2026

An Open-Source Normothermic Perfusion System Designed for Research Scientists
11:23

An Open-Source Normothermic Perfusion System Designed for Research Scientists

Published on: July 18, 2025

1.4K
Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
08:50

Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma

Published on: December 23, 2025

309
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
12:11

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

22.4K

Area of Science:

  • Biomedical Engineering
  • Nephrology
  • Fluid Dynamics

Background:

  • Continuous renal replacement therapy (CRRT) relies on hemofiltration (HF), but its long-term application is hindered by filter thrombosis and clogging.
  • Current HF systems typically have a maximum filter lifespan of less than 20 hours, necessitating frequent replacements.

Purpose of the Study:

  • To investigate the efficacy of outside-in hemofiltration (OIH) in achieving prolonged and consistent filter performance.
  • To analyze the impact of thrombus deposition on fluid flow and filtrate flux in the OIH system.

Main Methods:

  • Implementation of an outside-in hemofiltration setup with blood flowing into the inter-fiber space (IFS).
  • Microscopic examination of fiber bundle sections to assess thrombus morphology and distribution.
  • Development of a mathematical model to quantify the effect of thrombus deposition on hydrodynamic flow.

Main Results:

  • Achieved continuous hemofiltration for over 100 hours, a significant improvement over conventional methods.
  • Observed that thrombi in the IFS have minimal impact on blood flow and filtrate flux due to interconnected flow channels.
  • Demonstrated that thrombus dimensions are limited by inter-fiber gaps, preventing widespread clogging.

Conclusions:

  • Outside-in hemofiltration offers a promising solution for extending the lifespan of hemofiltration filters in renal replacement therapy.
  • The unique hydrodynamic properties of the IFS mitigate the negative effects of thrombus formation.
  • Further research into anticoagulant concentration is needed to optimize OIH performance, especially in cases of blood instability.