A novel biosensor array with a wheel-like pattern for glucose, lactate and choline based on electrochemiluminescence...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensing Technology

Background:

• Multiplex analysis requires sensitive and simultaneous detection methods.
• Electrochemiluminescence (ECL) imaging offers ultrasensitive detection capabilities.
• Enzyme-based biosensors are crucial for analyzing biological analytes.

Purpose of the Study:

• To develop a novel ECL imaging biosensor array for simultaneous determination of glucose, choline, and lactate.
• To utilize an enzyme/carbon nanotubes/chitosan composite film for enhanced biosensing performance.
• To demonstrate the potential applications of this platform in clinical diagnostics and food inspection.

Main Methods:

• Fabrication of an ECL imaging biosensor array using patterned indium tin oxide (ITO) glass and poly(dimethylsiloxane) (PDMS) covers.
• Modification of the biosensor array with an enzyme/carbon nanotubes/chitosan composite film.
• Capture of ECL images using a charge-coupled device (CCD) camera for simultaneous multi-analyte detection.

Main Results:

• The biosensor array successfully achieved simultaneous determination of glucose, choline, and lactate.
• Achieved detection limits of 14 μM for glucose, 40 μM for lactate, and 97 μM for choline.
• Demonstrated satisfactory performance in multicomponent assays and minimal cross-reactivity.

Conclusions:

• The developed ECL imaging biosensor array is a miniaturized, low-cost, and multi-functional platform.
• This novel biosensing platform shows significant potential for clinical diagnostics, medicine, and food inspection.
• ECL imaging provides a superior approach for ultrasensitive multiplex analysis in biosensing applications.