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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Apr 26, 2026

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

Patch-clamp technique in ESC-derived cardiomyocytes.

Jie Liu¹, Peter H Backx

¹Departments of Physiology and Medicine, University of Toronto, Medical Science Building, Rm 3247, 1 King's College Circle, Toronto, ON, Canada, M5S 1A8.

Methods in Molecular Biology (Clifton, N.J.)

|July 30, 2014

Summary

This summary is machine-generated.

This study demonstrates whole-cell patch clamp electrophysiology for characterizing human ether-à-go-go-related gene (hERG) currents in ESC-derived cardiomyocytes. The method effectively screens drug effects, like dofetilide, on these crucial cardiac ion channels.

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Last Updated: Apr 26, 2026

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

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Published on: August 4, 2022

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Area of Science:

Cardiology
Electrophysiology
Stem Cell Biology

Background:

Embryonic stem cell-derived cardiomyocytes (ESCs) exhibit electrophysiological properties similar to adult cardiomyocytes.
Ion channels are critical for cardiomyocyte function and drug response.
Patch-clamp electrophysiology is a key method for studying ion channel activity.

Purpose of the Study:

To illustrate the application of whole-cell patch clamp technique in ESC-derived cardiomyocytes.
To characterize human ether-à-go-go-related gene (hERG) currents and action potentials in these cells.
To evaluate the effects of hERG channel blockers on cardiomyocyte electrophysiology.

Main Methods:

Whole-cell patch clamp technique.
Recording of ionic currents and action potentials.
Pharmacological screening using hERG channel blocker dofetilide.

Main Results:

Successful recording of hERG currents and action potentials in ESC-derived cardiomyocytes.
Demonstration of dofetilide's effect on hERG channel activity.
Validation of patch-clamp for assessing drug modulation of cardiac ion channels.

Conclusions:

Whole-cell patch clamp is a robust method for studying ESC-derived cardiomyocyte electrophysiology.
This technique facilitates the assessment of ion channel function and drug interactions.
ESC-derived cardiomyocytes are a valuable model for cardiac research and drug development.