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Hydatidosis diagnosis by double diffusion in agar with arc 5 detection
Bulletin of the Pan American Health Organization
|January 1, 1989
Summary
The double diffusion arc 5 (DD5) test shows high specificity for diagnosing human hydatidosis, meaning positive results confirm infection. However, a negative DD5 result does not rule out the presence of hydatid cysts.
Area of Science:
- Medical Parasitology
- Immunodiagnostics
Background:
- Human hydatidosis remains a significant public health concern, necessitating accurate diagnostic tools.
- The double diffusion arc 5 (DD5) test is an immunodiagnostic method evaluated for its efficacy in detecting hydatidosis.
Purpose of the Study:
- To assess the diagnostic performance of the DD5 test for human hydatidosis.
- To determine the specificity and sensitivity of the DD5 test in a clinical setting.
Main Methods:
- Sera from 45 surgically confirmed hydatidosis patients and 54 patients with other pathologies were tested.
- Combined with previous data, the study analyzed 98 hydatidosis cases and 128 control cases.
- Positive results were defined by the presence of arc 5 or three or more precipitate bands.
Main Results:
- The DD5 test yielded positive results in 56% of confirmed hydatidosis cases (55/98).
- Seropositivity was lower in patients with pulmonary hydatidosis (36%).
- No false positive results were observed in the 128 control cases, indicating high specificity.
Conclusions:
- The DD5 test demonstrates high specificity for diagnosing hydatidosis in clinically selected individuals.
- A positive DD5 test result is highly indicative of hydatid infection.
- A negative DD5 test result should not be solely relied upon to exclude hydatidosis.