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Vibrationally resonant sum-frequency generation microscopy with a solid immersion lens.

Eun Seong Lee1, Sang-Won Lee2, Julie Hsu3

  • 1Center for Nanometrology, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 304-340, South Korea.

Biomedical Optics Express
|July 30, 2014
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Summary

We developed a new microscopy technique using a sapphire lens to achieve high-resolution imaging. This method enhances optical performance for detailed visualization of microscopic structures.

Keywords:
(110.3080) Infrared imaging(170.0180) Microscopy(180.4315) Nonlinear microscopy(190.4223) Nonlinear wave mixing

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Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Spectroscopy

Background:

  • Vibrationally resonant sum-frequency generation (VR-SFG) microscopy offers molecular specificity.
  • Achieving high spatial resolution in mid-infrared VR-SFG microscopy is challenging.
  • Immersion microscopy enhances resolution by increasing the numerical aperture (NA).

Purpose of the Study:

  • To demonstrate high-resolution VR-SFG microscopy in the mid-infrared range.
  • To enhance the NA of a parabolic mirror objective using a hemispheric sapphire lens.
  • To evaluate the practical utility of sapphire immersion for biological tissue imaging.

Main Methods:

  • Utilized a hemispheric sapphire lens as a solid immersion medium.
  • Combined the sapphire lens with an off-axis parabolic mirror objective.
  • Performed VR-SFG microscopy and imaged collagen-rich tissues.

Main Results:

  • Enhanced the NA from 0.42 to 0.72, a 1.72-fold increase.
  • Achieved a lateral resolution as high as 0.64 μm.
  • Demonstrated successful imaging of collagen-rich tissues using the sapphire immersion lens.

Conclusions:

  • A hemispheric sapphire lens effectively enhances NA for mid-infrared VR-SFG microscopy.
  • Sapphire immersion microscopy significantly improves lateral resolution.
  • This technique is practical for high-resolution imaging of biological tissues.