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Updated: Apr 26, 2026

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
Published on: November 30, 2022
A portable lipid bilayer system for environmental sensing with a transmembrane protein
Ryuji Kawano1, Yutaro Tsuji2, Koki Kamiya1
1Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan.
Abstract:
This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.
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