[Tissue culturing and ultrastructural observation of human trabecular cells]

Two trabecular cell lines were established by means of tissue culturing. The growth patterns of the trabecular cell culture were studied under phase-contrast microscope for 5 generations. The morphological features and the ultrastructure of the trabecular cells were investigated by light microscopy, fluorescence microscopy, and scanning and transmission electron microscopy. The cultured cells were identified as trabecular cells by their specific characteristics quite different from the possible contaminating cells, e.g. fibrocytes, corneal endothelium, etc., and they may be used in pathogenetical and pharmacological researches of glaucoma.