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A neutron dynamic therapy with a boron tracedrug UTX-51 using a compact neutron generator
Hitoshi Hori1, Ryu Tada2, Yoshihiro Uto2
1Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima, Tokushima, Japan hori@bio.tokushima-u.ac.jp hitoshi.hori@gmail.com.
Background/Aim:
We are developing a neutron dynamic therapy (NDT) with boron tracedrugs for a new mechanical-clearance treatment of pathotoxic misfolded, aggregated, and self-propagating prion-associated disease proteins. We present a compact neutron generator-based NDT using a boron tracedrug UTX-51. Our NDT is based on the weak thermal neutron-bombarded destructive action of UTX-51 on bovine serum albumin (BSA) using the neutron beams produced from a compact inertial electrostatic confinement fusion (IECF) neutron generator.
Results:
BSA as an NDT molecular target was subjected to thermal neutron irradiation for eight hours using a compact neutron generator. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern showed no protein band when 2 nmoles of BSA were irradiated with more than 100 nmoles of UTX-51, while BSA was not affected when irradiated without UTX-51.
Conclusion:
For the first time, we have succeeded in the molecular destruction of a prion-disease model protein, BSA, by NDT with a boron tracedrug, UTX-51, using a compact neutron generator.