Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

135
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
135
X-ray Crystallography02:18

X-ray Crystallography

21.5K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
21.5K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ structures of the portal-neck-tail complex of bacteriophage T4 inform a viral genome positioning mechanism.

Nature communications·2026
Same author

Structure of a human Rhinovirus complexed with its receptor molecule.

Protein engineering·2024
Same author

Structure of <i>Vibrio</i> Phage XM1, a Simple Contractile DNA Injection Machine.

Viruses·2023
Same author

Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1.

Nature communications·2022
Same author

Structures of a large prolate virus capsid in unexpanded and expanded states generate insights into the icosahedral virus assembly.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Cryo-EM structures of alphavirus conformational intermediates in low pH-triggered prefusion states.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
See all related articles

Related Experiment Video

Updated: Apr 26, 2026

Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

4.1K

Serial crystallography using synchrotron radiation.

Michael G Rossmann1

  • 1Department of Biological Sciences, Purdue University , West Lafayette, IN 47907, USA.

Iucrj
|July 31, 2014
PubMed
Summary
This summary is machine-generated.

X-ray diffraction data recording has evolved significantly. Powerful femtosecond X-ray pulses from free electron lasers now offer new possibilities for crystal structure analysis.

Keywords:
in vivo-grown microcrystalsprotein microcrystallographyserial crystallography

More Related Videos

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

2.8K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

16.0K

Related Experiment Videos

Last Updated: Apr 26, 2026

Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

4.1K
Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

2.8K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

16.0K

Area of Science:

  • Crystallography
  • X-ray physics

Background:

  • Historical methods of X-ray diffraction data collection from crystals are reviewed.
  • The evolution of X-ray sources has been crucial for advancements in structural biology and materials science.

Purpose of the Study:

  • To provide a historical overview of X-ray diffraction data recording techniques.
  • To highlight the impact of emerging technologies, such as free electron lasers, on X-ray diffraction.

Main Methods:

  • Review of historical literature on X-ray diffraction data acquisition.
  • Discussion of the characteristics and capabilities of free electron lasers for X-ray generation.

Main Results:

  • X-ray diffraction data recording has transitioned from early photographic methods to modern digital detectors.
  • Free electron lasers provide unprecedented X-ray pulse intensity and duration (femtoseconds).

Conclusions:

  • Modern X-ray sources like free electron lasers are revolutionizing the field of X-ray diffraction.
  • These advancements open new avenues for studying crystal structures with enhanced resolution and speed.