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Updated: Apr 26, 2026
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Published on: January 3, 2014
Development of new potato virus X-based vectors for gene over-expression and gene silencing assay
Ying Wang1, Qian-Qian Cong2, Yu-Fei Lan3
1Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Shandong 271018, China; Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Shandong 271018, China.
Abstract:
Multiple plant viruses, including potato virus X (PVX), have been modified as vectors for expressing heterologous genes or silencing endogenous genes in plants. PVX-based vectors facilitate the functional analysis of genes in plant. However, they can only express one protein in a time. In this paper we report the construction of new vectors based on a 35S promoter-driven PVX infectious clone, pCaPVX100. Vector pCaPVX440 contains two additional subgenomic promoters and can be utilized to express two foreign genes at the same time. Plasmid pCaPVX760 is a CP minus vector and can be used to express foreign proteins through the gene substitution strategy. In addition, plasmid pCaPVX100 was engineered into a gene silencing vector (pCaPVX440-LIC) by introducing a ligation independent cloning (LIC) site into the vector. These results indicate that the newly developed PVX vectors are competent for multiple research purposes.
