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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
Published on: August 9, 2013
mRNA decay factor AUF1 binds the internal ribosomal entry site of enterovirus 71 and inhibits virus replication
Jing-Yi Lin1, Mei-Ling Li2, Gary Brewer2
1School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.
Abstract:
AU-rich element binding factor 1 (AUF1) has a role in the replication cycles of different viruses. Here we demonstrate that AUF1 binds the internal ribosome entry site (IRES) of enterovirus 71 (EV71) and negatively regulates IRES-dependent translation. During EV71 infection, AUF1 accumulates in the cytoplasm where viral replication occurs, whereas AUF1 localizes predominantly in the nucleus in mock-infected cells. AUF1 knockdown in infected cells increases IRES activity and synthesis of viral proteins. Taken together, the results suggest that AUF1 interacts with the EV71 IRES to negatively regulate viral translation and replication.
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