Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Translating Human Prototype Liver Implant Technology from Academia to Industry for Third-Party Transplant and In Vivo Validation.

Cells·2026

Same author

Glycosaminoglycans in tissue regeneration: Insights into glycobiology and their biomedical application.

Bioactive materials·2026

Same author

Radiology-based artificial intelligence for predicting targeted therapy response in pan-cancer: a comprehensive review.

Journal of translational medicine·2025

Same author

Ultrafast crosslinking, strongly adhesive <i>de novo</i> protein hydrogels promote cartilage regeneration.

Bioactive materials·2025

Same author

Transcriptomic Profiling of iPS Cell-Derived Hepatocyte-like Cells Reveals Their Close Similarity to Primary Liver Hepatocytes.

Cells·2025

Same author

Genome editing of TXNIP in human pluripotent stem cells for the generation of hepatocyte-like cells and insulin-producing islet-like aggregates.

Stem cell research & therapy·2025

Same journal

Reprogramming Urine-Derived Cells using Commercially Available Self-Replicative RNA and a Single Electroporation.

Current protocols in stem cell biology·2020

Same journal

Human iPSC-Derived Blood-Brain Barrier Models: Valuable Tools for Preclinical Drug Discovery and Development?

Current protocols in stem cell biology·2020

Same journal

Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells.

Current protocols in stem cell biology·2020

Same journal

From Hair to iPSCs-A Guide on How to Reprogram Keratinocytes and Why.

Current protocols in stem cell biology·2020

Same journal

Simple Workflow and Comparison of Media for hPSC-Cardiomyocyte Cryopreservation and Recovery.

Current protocols in stem cell biology·2020

Same journal

Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research.

Current protocols in stem cell biology·2020

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 26, 2026

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Published on: March 2, 2017

Deriving functional hepatocytes from pluripotent stem cells.

Dagmara Szkolnicka¹, Sarah L Farnworth, Baltasar Lucendo-Villarin

¹MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom.

Current Protocols in Stem Cell Biology

|August 2, 2014

Summary

This summary is machine-generated.

Researchers developed a new method to create human liver cells from pluripotent stem cells. This highly efficient technique yields active, drug-inducible cells, advancing liver disease research and potential cell-based therapies.

Keywords:

cell-based therapies hepatocyte human embryonic stem cells liver models ‘in a dish’pluripotent stem cells

More Related Videos

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

Related Experiment Videos

Last Updated: Apr 26, 2026

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Published on: March 2, 2017

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

Area of Science:

Stem cell biology
Hepatology
Regenerative medicine

Background:

Liver transplantation is the only cure for end-stage liver disease but is limited by organ availability.
Cell-based therapies and in vitro models are crucial for advancing liver disease research and treatment.
Developing reliable human liver cell models is essential for understanding disease mechanisms and discovering new therapies.

Purpose of the Study:

To develop efficient methodologies for differentiating pluripotent stem cells into functional human hepatocytes.
To create predictive in vitro models for studying liver disease and drug responses.
To advance cell-based therapeutic strategies for liver failure.

Main Methods:

Utilized pluripotent stem cells for directed differentiation into hepatocytes.
Optimized differentiation protocols to achieve high efficiency and cell activity.
Validated the functionality and drug-inducibility of the derived human hepatocytes.

Main Results:

Achieved approximately 90% efficiency in hepatocyte differentiation.
Generated human hepatocyte populations that are active and drug-inducible.
Demonstrated the predictive potential of these cells for drug responses and disease modeling.

Conclusions:

The developed hepatocyte differentiation method is robust and highly efficient.
These derived human hepatocytes offer a valuable tool for liver disease research and drug discovery.
This advancement supports the development of novel cell-based therapies for liver failure.