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Updated: Apr 26, 2026

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
Published on: August 18, 2023
The chaperonin CCTα is required for efficient transcription and replication of rabies virus
Jinyang Zhang1, Chengjin Ye, Xizhen Ruan
1Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058; State Key Laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou, 310003; Research Center of Molecular Medicine of Yunnan Province, Kunming University of Science and Technology, Kunming, 650500, China.
Abstract:
Negri bodies (NBs) are formed in the cytoplasm of rabies virus (RABV)-infected cells and are accompanied by a number of host factors to NBs, in which replication and transcription occur. Here, it was found that chaperonin containing TCP-1 subunit alpha (CCTα) relocalizes to NBs in RABV-infected cells, and that cotransfection of nucleo- and phospho-proteins of RABV is sufficient to recruit CCTα to the NBs' structure. Inhibition of CCTα expression by specific short hairpin RNA knockdown inhibited the replication and transcription of RABV. Therefore, this study showed that the host factor CCTα is associated with RABV infection and is very likely required for efficient virus transcription and replication.
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