Related Experiment Video
Updated: Apr 26, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
Published on: May 20, 2013
Angle of incidence averaging in reflectance measurements with optical microscopes for studying layered
Nihit Saigal1, Amlan Mukherjee1, Vasam Sugunakar1
1Department of Condensed Matter Physics and Materials Science, Tata Institute of Fundamental Research, Mumbai 400 005, India.
Abstract:
Reflectance spectrum measured using an optical microscope with a large numerical aperture objective lens is shown to get modified. The change is most prominent when there are optical interference related features in the spectrum. This modification is shown to arise primarily due to the wide range of angles of incidence involved in the measurement and a simple formulation is provided to correct for this in simulations. The importance of such analysis is brought out through a reflectance contrast spectroscopy based study for identifying mono-layer and bi-layer graphene and MoS2.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...

