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Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures
Published on: October 17, 2008
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Purification and culture of dorsal root ganglion neurons
1Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305.
Cold Spring Harbor Protocols
|August 3, 2014
Abstract:
Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.
