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Updated: Apr 26, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Improved enantioseparation via the twin-column based recycling high performance liquid chromatography
Qi Liu1, Junan Xiao1, Jingang Yu1
1College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Abstract:
A long-neglected recycling high performance liquid chromatography (R-HPLC) strategy was adopted to improve the enantioseparation efficiency in this work. Taking intractable isoxazolidine mixtures as the example, two different R-HPLC methods including single-column and twin-column based strategy were applied to improve the enantioseparation efficiency. Under the optimized conditions, these two methods present distinct separation results: the conceptually simple and commonly used single-column based R-HPLC led to severe peak broadening and degraded separation efficiency; on the contrary, the enantioseparation was significantly improved by adopting the relatively complicated twin-column strategy. This observed difference might be mainly attributed to less peak spreading and sample mixing of the latter, as a result of avoiding redirecting the effluent back to LC pump.
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