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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Published on: May 2, 2018
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Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis
M N Zakaria1,2,3, T Takeshita1, Y Shibata1
1Section of Preventive and Public Health Dentistry, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
International Endodontic Journal
|August 5, 2014
Summary
Microbial analysis of persistent periapical lesions revealed diverse bacteria, with specific patterns in symptomatic cases. Bacterial communities, not single species, likely drive persistent inflammation in root-filled teeth.
Area of Science:
- Microbiology
- Oral Pathology
- Molecular Genetics
Background:
- Persistent periapical lesions are common complications of root-filled teeth.
- Understanding the microbial etiology is crucial for effective treatment.
- Previous studies have suggested various bacteria, but comprehensive molecular analysis is needed.
Purpose of the Study:
- To characterize the microbial composition of persistent periapical lesions using molecular genetics.
- To identify specific bacterial patterns associated with symptomatic versus asymptomatic lesions.
Main Methods:
- DNA extraction from apical lesion samples of 12 patients undergoing apicectomy.
- 16S rRNA gene clone library analysis for comprehensive microbial profiling.
- Polymerase chain reaction (PCR) for specific detection of Enterococcus faecalis, Candida albicans, and Porphyromonas gingivalis fimA genotypes.
Main Results:
- Bacteria were present in all samples, dominated by P. gingivalis, Fusobacterium nucleatum, and Propionibacterium acnes.
- Symptomatic lesions exhibited greater bacterial diversity and distinct microbial communities, including Prevotella spp., Treponema spp., and specific P. gingivalis genotypes.
- Enterococcus faecalis and Candida albicans were not detected in any samples.
Conclusions:
- Persistent periapical lesions harbor diverse bacterial communities.
- Distinct microbial patterns characterize symptomatic lesions, suggesting a community-based etiology.
- Bacterial combinations, rather than individual species, may be responsible for persistent periapical inflammation.
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