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Updated: Apr 26, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Insulin-Based Regulation of Glucose-functionalized Nanoparticle Uptake in Muscle Cells
Yi-Cheun Yeh1, Sung Tae Kim1, Rui Tang1
1Department of Chemistry, University of Massachusetts at Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA, Tel.: (+1) 413-545-2058;
Abstract:
Effective regulation of nanoparticle (NP) uptake facilitates the NP-based therapeutics and diagnostics. Here, we report the use of insulin and 2-deoxyglucose (2-DG) to modulate the cellular uptake of glucose-functionalized quantum dots (Glc-QDs) in C2C12 muscle cells. The cellular uptake of Glc-QDs can be modulated up to almost two-fold under insulin stimulation while be down-regulated in the presence of 2-DG. These results demonstrate the use of secondary regulators to control the cellular uptake of NPs through membrane protein recognition in a specific and fine-tunable fashion.
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