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Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue
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Characterisation of skin barrier function using bioengineering and biophysical techniques.

Quan Yang1, Richard H Guy

  • 1Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK.

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Summary

Forehead skin barrier function is less effective than forearm skin. This study used bioengineering tools to compare skin barrier properties at these two sites in healthy volunteers.

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Area of Science:

  • Dermatology
  • Bioengineering
  • Biophysics

Background:

  • Skin barrier function is crucial for protection.
  • Stratum corneum (SC) and its lipids are key components of the skin barrier.
  • Understanding regional differences in skin barrier function is important.

Purpose of the Study:

  • To characterize in vivo skin barrier function at two distinct anatomic sites (forearm and forehead).
  • To utilize minimally invasive bioengineering and biophysical tools for quantitative assessment.
  • To compare the integrity and composition of the stratum corneum between the forearm and forehead.

Main Methods:

  • Quantified stratum corneum (SC) quantity using gravimetric and imaging techniques.
  • Assessed SC intercellular lipid organization with attenuated total reflectance infrared spectroscopy (ATR-IR).
  • Identified and quantified natural moisturizing factor (NMF) components using liquid chromatography-mass spectrometry.

Main Results:

  • SC was thinner and less organized on the forehead compared to the forearm.
  • Intercellular lipids were more disordered on the forehead.
  • While NMF composition was similar, forehead SC yielded less total NMF.

Conclusions:

  • Forehead skin barrier function is less competent than forearm skin barrier function.
  • Bioengineering and biophysical techniques provide objective, quantitative data on skin barrier differences.
  • These findings highlight regional variations in skin barrier properties.