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Updated: Apr 26, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Monolithic silica columns functionalized with substituted polyproline-derived chiral selectors as chiral stationary
Raquel Sancho1, Arnau Novell, Frantisek Svec
1Food and Nutrition Torribera Campus, University of Barcelona, Santa Coloma de Gramenet, Barcelona, Spain; Laboratori de Química Farmacèutica, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
Abstract:
In this study, two polyproline-derived chiral selectors are bonded to monolithic silica gel columns. In spite of high chiral selector coverage, the derivatization was found to have only a slight effect on the hydrodynamics of the mobile phase through the column. The enantioseparation ability of the resulting chiral monolithic columns was evaluated with a series of structurally diverse racemic test compounds. When compared to analogous bead-based chiral stationary phases, higher enantioseparation and broader application domain were observed for monolithic columns. Moreover, the increase in flow rate produces a minor reduction of resolution, which permits to shorten analysis time. Additionally, increased loadability defines chiral polyproline derived monoliths as adequate for preparative chromatography.
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