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A high-performance liquid chromatography method for the simultaneous assay of diaminopimelate epimerase and
1Institute for Biotechnological Studies, University of Kent, Canterbury, United Kingdom.
Analytical Biochemistry
|August 1, 1989
Abstract:
A sensitive and comparatively simple method for the assay of diaminopimelate (DAP) decarboxylase, which simultaneously monitors DAP epimerase activity, in the reverse of the biosynthetic direction, is described. The substrate, meso-DAP and products LL-DAP and L-lysine are derivatized with o-phthaldialdehyde and resolved by reversed-phase high-performance liquid chromatography. Separation is achieved on a Spherisorb C18 column using a gradient elution system. This technique offers a high degree of sensitivity as the detection method described can measure picomole quantities of substrate and products.