Applicability of UV laser-induced solid-state fluorescence spectroscopy for characterization of solid dosage forms

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Pharmaceutical Technology

Background:

• High-throughput pharmaceutical manufacturing demands rapid quality control methods.
• Fast analytical techniques are also essential in forensic science for preliminary investigations.

Purpose of the Study:

• To design and optimize a setup for rapid, non-invasive tablet characterization using laser-induced fluorescence spectroscopy.
• To validate the setup's performance and investigate the influence of tablet properties on spectral data.

Main Methods:

• Utilized a UV-laser (266 nm) in reflection geometry for laser-induced fluorescence spectroscopy.
• Validated the setup for repeatability, bleaching, and sensitivity.
• Investigated effects of hardness, homogeneity, composition, and granule size on spectra using Design of Experiments.
• Applied multiplicative scatter correction for spectral data preprocessing.
• Employed principal component analysis for discriminating tablets based on fluorescence spectra.

Main Results:

• The laser-induced fluorescence spectroscopy setup demonstrated good repeatability and sensitivity.
• Multiplicative scatter correction effectively preprocessed the fluorescence spectra.
• Tablets with varying physical and chemical properties were successfully discriminated using principal component analysis of their fluorescence spectra.

Conclusions:

• The developed laser-induced fluorescence spectroscopy method offers a rapid and non-invasive approach for tablet quality control.
• The technique is sensitive to physical and chemical variations in tablets, enabling effective discrimination.
• This method holds potential for applications in pharmaceutical quality assurance and forensic analysis.