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Updated: Apr 26, 2026
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
85.8K
Ten years of next-generation sequencing technology
Erwin L van Dijk1, Hélène Auger1, Yan Jaszczyszyn2
1Centre de Génétique Moléculaire - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
Trends in Genetics : TIG
|August 11, 2014
Summary
Next-generation sequencing (NGS) has rapidly advanced in speed, cost, and capability over the past decade. These improvements have democratized NGS, enabling widespread applications in science, medicine, and forensics.
Area of Science:
- Genomics
- Biotechnology
- Molecular Biology
Background:
- Next-generation sequencing (NGS) technologies have undergone significant evolution over the last ten years.
- Key advancements include increased speed, longer read lengths, higher throughput, and reduced per-base costs.
Purpose of the Study:
- To provide an overview of the evolution of NGS technologies.
- To discuss significant improvements in sequencing and library preparation.
- To explore current NGS applications and future perspectives.
Main Methods:
- Review of technological advancements in sequencing.
- Analysis of library preparation protocol improvements.
- Survey of current and emerging NGS applications.
Main Results:
- Democratization of NGS due to technological progress.
- Development of novel NGS applications in basic and translational research.
- Expansion of NGS use in clinical diagnostics, agrigenomics, and forensic science.
Conclusions:
- NGS technology has matured significantly, becoming more accessible and versatile.
- Continued innovation in NGS promises further breakthroughs across scientific disciplines.
- The impact of NGS is profound, driving progress in diverse research and diagnostic fields.
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