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Updated: Apr 26, 2026

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA

Published on: February 12, 2020

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Nandrolone decanoate is able to modulate proliferation and adhesion of myoblasts

E N Oliveira, K P Fernandes, C A Silva

    Endocrine Regulations
    |August 12, 2014
    PubMed

    Insights

    Nandrolone decanoate (ND) enhanced muscle cell adhesion and proliferation in vitro. This anabolic steroid may aid muscle injury repair by promoting cell growth and attachment.

    Area of Science:

    • Muscle regeneration and repair
    • Cellular biology
    • Pharmacology

    Background:

    • Muscle injuries necessitate effective repair strategies.
    • Anabolic steroids are investigated for their potential therapeutic effects.
    • Understanding cellular mechanisms is key to optimizing muscle healing.

    Purpose of the Study:

    • To investigate the impact of nandrolone decanoate (ND) on C2C12 myoblast cells.
    • To assess ND's effects on cell proliferation, adhesion, and myogenic regulatory factor (MRF) expression.

    Main Methods:

    • MTT assay used to measure cell proliferation and adhesion.
    • Real-time PCR employed to quantify MRF gene expression.
    • C2C12 myoblasts treated with varying concentrations of ND.

    Main Results:

    • ND at 10 or 25 µM increased cell adhesion within 60 minutes.
    • ND at 5 µM promoted cell proliferation after 5 days.
    • ND at 50 µM decreased cell proliferation after 5 days; MRF expression remained unchanged.

    Conclusions:

    • Nandrolone decanoate demonstrates dose-dependent effects on C2C12 cell proliferation.
    • ND enhances C2C12 cell adhesion and proliferation, suggesting potential benefits for muscle injury.
    • The steroid does not appear to influence the expression of key myogenic regulatory factors.

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