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Published on: June 28, 2011
Improved synthesis of [(18)F]FLETT via a fully automated vacuum distillation method for [(18)F]2-fluoroethyl azide
Uwe Ackermann1, Lucie Plougastel2, Yit Wooi Goh3
1Department of Nuclear Medicine and Centre for PET, Austin Health, Heidelberg, VIC, Australia; The University of Melbourne, Parkville, VIC, Australia; Ludwig Institute for Cancer Research, Melbourne Branch, VIC, Australia.
Abstract:
The synthesis of [(18)F]2-fluoroethyl azide and its subsequent click reaction with 5-ethynyl-2'-deoxyuridine (EDU) to form [(18)F]FLETT was performed using an iPhase FlexLab module. The implementation of a vacuum distillation method afforded [(18)F]2-fluoroethyl azide in 87±5.3% radiochemical yield. The use of Cu(CH3CN)4PF6 and TBTA as catalyst enabled us to fully automate the [(18)F]FLETT synthesis without the need for the operator to enter the radiation field. [(18)F]FLETT was produced in higher overall yield (41.3±6.5%) and shorter synthesis time (67min) than with our previously reported manual method (32.5±2.5% in 130min).
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