Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Magnetic DNA walker mediated in-situ deposition of polyaniline for the electrochemical detection of kanamycin.

Bioelectrochemistry (Amsterdam, Netherlands)·2026

Same author

Human umbilical cord mesenchymal stem cell-derived nanovesicles ameliorate acute lung injury by hsa-let-7g-5p inhibition of NF-κB/NLRP3 pathway.

Extracellular vesicles and circulating nucleic acids·2026

Same author

CRISPR/Cas13a-Driven Catalytic Hairpin Assembly of a Quantum Dot Nanobeacon for Viral RNA Detection.

Analytical chemistry·2026

Same author

A novel steady-state chemiluminescent aptasensor using rolling circle amplification products DNA hydrogel for rapid and ultra-sensitive detection of Salmonella typhimurium in chicken.

Mikrochimica acta·2026

Same author

An autosampling microfluidic chip enables FEN1-aided recombinase polymerase amplification to rapidly detect multiple urinary tract infection pathogens on-site.

Mikrochimica acta·2026

Same author

Simultaneous detection of SARS-CoV-2 proteins and nucleic acids by coupling PNIPAM with circle-to-circle amplification.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026

Search research articles

Related Experiment Video

Updated: Apr 25, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Periodic fluorescent silver clusters assembled by rolling circle amplification and their sensor application.

Tai Ye¹, Jinyang Chen, Yufei Liu

¹Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, P. R. China.

ACS Applied Materials & Interfaces

|August 14, 2014

Summary

Researchers developed DNA-stabilized silver nanocluster (Ag NCs) nanowires for sensitive DNA detection. These novel nanowires offer high sensitivity and potential for clinical imaging applications.

Keywords:

rolling circle amplification self-assembly silver cluster

More Related Videos

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Related Experiment Videos

Last Updated: Apr 25, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Area of Science:

Nanotechnology
Biochemistry
Molecular Biology

Background:

DNA-templated silver nanoclusters (Ag NCs) offer unique optical properties.
Developing stable and sensitive nanostructures for biomolecular detection is crucial.

Purpose of the Study:

To present a simple method for preparing DNA-stabilized Ag NCs nanowires.
To evaluate the potential of these nanowires for sensitive DNA detection and imaging.

Main Methods:

Fabrication of Ag NCs nanowires using two unmodified DNA strands and an enzymatic scaffold.
Assembly of fluorescent Ag NCs into three-way junction (TWJ) structures within the nanowires.
Visualization using confocal microscopy and assessment of DNA detection sensitivity via rolling circle amplification (RCA).

Main Results:

Successfully prepared DNA-stabilized Ag NCs nanowires at room temperature.
Demonstrated clear visualization of nanowires using confocal microscopy.
Achieved high sensitivity for specific DNA detection with a wide linear range (6–300 pM) and a low detection limit (0.84 pM) in complex serum samples.

Conclusions:

The developed Ag NCs nanowires are a promising platform for sensitive DNA detection.
The method is simple and utilizes readily available components.
These nanowires show significant potential for future clinical diagnostics and bioimaging applications.