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Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Apr 25, 2026

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Published on: August 22, 2007

Lectin-probed western blot analysis.

¹Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan, taksato@vos.nagaokaut.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|August 14, 2014

Summary

This summary is machine-generated.

Lectin blot analysis directly reveals glycoprotein glycan structures without glycan release. This method, combined with glycosidase digestion, offers enhanced insights into carbohydrate-binding specificities.

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Last Updated: Apr 25, 2026

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

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Published on: August 22, 2007

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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

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Published on: September 28, 2018

Area of Science:

Biochemistry
Glycobiology
Molecular Biology

Background:

Glycoproteins play crucial roles in various biological processes.
Understanding glycan structures is essential for deciphering glycoprotein functions.
Existing methods for glycan analysis can be complex and time-consuming.

Purpose of the Study:

To describe protocols for standard lectin blot analysis.
To demonstrate the application of lectin blot analysis in characterizing glycan structures.
To present the combined use of lectin blot analysis with glycosidase digestion for deeper insights.

Main Methods:

Lectin-probed western blot analysis (lectin blot analysis).
Direct analysis of glycan structures on glycoproteins.
Enzymatic digestion of blots using glycosidases.

Main Results:

Lectin blot analysis provides direct information on glycan structures.
The method allows for the characterization of carbohydrate-binding specificities.
Combining lectin blot analysis with glycosidase digestion enhances structural elucidation.

Conclusions:

Lectin blot analysis is a valuable technique for basic glycan structure determination.
The described protocols facilitate direct glycan analysis without prior release.
Glycosidase digestion in conjunction with lectin blotting offers a powerful approach for glycan profiling.