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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
Surface plasmon resonance as a tool to characterize lectin-carbohydrate interactions
Yasuro Shinohara1, Jun-ichi Furukawa
1Laboratory of Medical and Functional Glycomics, Graduate School of Advanced Life Science, and Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, Sapporo, 001-0021, Japan, yshinohara@sci.hokudai.ac.jp.
Surface plasmon resonance (SPR) biosensors offer label-free, real-time analysis of molecular interactions, particularly lectin-carbohydrate binding. This technology enables detailed characterization and screening for various applications, including diagnostics.
Area of Science:
- Biophysical Chemistry
- Analytical Chemistry
- Biochemistry
Background:
- Surface plasmon resonance (SPR) biosensors detect real-time changes in refractive index near a surface.
- This label-free technique facilitates rapid molecular interaction characterization.
- SPR is valuable for analyzing binding kinetics, thermodynamics, and concentrations in complex samples.
Purpose of the Study:
- To detail the applications of SPR biosensors in analyzing lectin-carbohydrate interactions.
- To highlight SPR's utility in characterizing specificity, affinity, kinetics, and stoichiometry.
- To emphasize SPR's role in screening interactions for diagnostics and identifying ligands.
Main Methods:
- Utilizing SPR biosensors for real-time, label-free monitoring of molecular binding events.
- Quantitative analysis of binding affinities ranging from millimolar to picomolar.
- Employing SPR for detailed interaction characterization and high-throughput screening.
Main Results:
- SPR enables sensitive analysis of molecular interactions, with typical analyte concentration ranges of 0.1-100×Kd.
- The method requires small sample volumes, typically 50-150 μL.
- SPR facilitates both in-depth analysis and broad screening of lectin-carbohydrate interactions.
Conclusions:
- SPR biosensors are powerful tools for characterizing lectin-carbohydrate interactions with high sensitivity and specificity.
- The method supports diverse applications, from fundamental binding studies to diagnostic screening.
- While solid-phase binding has complexities, SPR can mimic biological conditions like cell-cell interactions.
