Carbohydrate-lectin interaction assay by fluorescence correlation spectroscopy using fluorescence-labeled...

Fluorescence correlation spectroscopy (FCS) is a high-throughput system for the assay of interactions in solution and can be used to measure the numbers of molecules and molecular size in micro-regions. FCS can be used to measure interactions in environments that are close to those in vivo. It is a useful technique for measuring bioactive substances, screening inhibitors, and detecting the binding of materials, as well as for determining K d and IC50 values. Glycosyl amino acids with natural oligosaccharides are useful for the interaction assay of oligosaccharides. Fluorescence probes can be introduced into the glycosyl amino acid while the whole structure of the oligosaccharide is maintained. Carbohydrate-lectin interaction in a solution assay system can be analyzed easily by FCS using fluorescence-labeled glycosylasparagine.