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Updated: Apr 25, 2026

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

Published on: December 25, 2021

The first gamma-H2AX biodosimetry intercomparison exercise of the developing European biodosimetry network RENEB.

S Barnard¹, E A Ainsbury², J Al-hafidh²

¹Public Health England, Centre for Radiation Chemical and Environmental Hazards, Chilton, UK stephen.barnard@phe.gov.uk.

Radiation Protection Dosimetry

|August 14, 2014

Summary

The gamma-H2AX foci assay offers rapid radiation dose estimation after incidents. This biodosimetry tool helps triage individuals by identifying those most severely exposed for further assessment.

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Published on: July 3, 2015

Area of Science:

Radiation biology
Biophysical sciences
Medical physics

Background:

Mass casualty radiation incidents pose significant public health challenges.
Accurate and rapid biodosimetry is crucial for effective response and medical management.
The gamma-H2AX foci assay is a promising biomarker for radiation exposure.

Purpose of the Study:

To evaluate the utility of the gamma-H2AX foci assay for rapid radiation dose estimation in a network setting.
To assess the feasibility of the gamma-H2AX assay for early triage categorization of exposed individuals.
To establish the reliability of the gamma-H2AX assay across multiple European laboratories.

Main Methods:

A European-wide exercise involving 8 laboratories within the RENEB framework.
Telescoring comparison of 200 gamma-H2AX foci images from 8 samples.
Comparison of fresh blood lymphocyte samples analyzed 4 and 24 hours post-shipment.

Main Results:

The gamma-H2AX foci assay demonstrated rapid processing times (hours) and potential for high-throughput batch analysis.
Significant variations in dose-response relationships were observed between participating laboratories.
Despite variations, the assay network showed capability in identifying severely exposed individuals.

Conclusions:

The gamma-H2AX foci assay is a valuable tool for rapid biodosimetry in mass casualty radiation incidents.
The assay facilitates early triage by distinguishing between worried well and exposed individuals.
Further standardization may improve inter-laboratory consistency for dose assessment.