Proteins immobilization on the surface of modified plant viral particles coated with hydrophobic polycations
Nikolai A Nikitin1, Andrei S Malinin, Ekaterina A Trifonova
1a Faculty of Biology , Lomonosov Moscow State University , Moscow , Russia.
Abstract:
Two hydrophobic cations based on poly-N-ethyl-vinylpyridine were used to produce biologically active complexes. The complexes obtained from tobacco mosaic virus (TMV) spherical particles (SPs), hydrophobic polycation, and a model protein were stable and did not aggregate in solution, particularly at high ionic strengths. The nucleic acid-free SPs were generated by thermal remodeling of the TMV (helical rod-shaped plant virus). The model protein preserved its antigenic activity in the ternary complex (SP-polycation-protein). Immobilization of proteins on the surface of SPs coated with hydrophobic cation is a promising approach to designing biologically active complexes used in bionanotechnologies.
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