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Repression of sporulation: isolation and characterization of repression-resistant mutants of Bacillus subtilis
S Bhaduri1, S Bandyopadhyay, S K Bose
1Department of Biochemistry, University College of Science, Calcutta, India.
The Journal of Applied Bacteriology
|November 1, 1989
Abstract:
The sporulation of Bacillus subtilis B34 was repressed by 24 h if glutamine or ammonium chloride but not glutamate was added at late log phase (70 h) when glucose and glutamate were nearly exhausted. Glutamine-resistant mutants were isolated by selective heat treatment during the delay period induced by glutamine. Glutamine-resistant mutants showed cross resistance not only against ammonium chloride but also against glucose just as glucose-resistant mutants showed resistance against nitrogenous catabolites.