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Updated: Apr 25, 2026
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
Published on: October 13, 2021
Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell
J Lei1, L T McLane2, J E Curtis3
1Woodruff School of Mechanical Engineering. Georgia Institute of Technology, Atlanta, Georgia, USA.
Abstract:
Mesenchymal stem cells therapies have the potential to treat many pathologies, however, controlling cell fate after implantation remains challenging. We have used a multilayer technology to graft a range of 5 μg/mL - 5 mg/mL heparin onto the surface of MSC aggregates. Heparin coating does not affect cell viability (seen through LIVE/DEAD staining), cell anti-inflammatory properties (seen through co-culture with activated monocytes)and facilitates sequestration by coated cells of a growth factor (TGF-β1) that remains bioactive. This system can maximize therapeutic potential of MSC-based treatments because the cell surface-loaded protein could both signal to the cells to influence transplanted cell fate and be released into the surrounding environment to help repair injured tissue.
