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Updated: Apr 25, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
Published on: May 9, 2020
mTOR regulates the nucleoplasmic diffusion of Xrn2 under conditions of heat stress
Kazunori Watanabe1, Kenichi Ijiri2, Takashi Ohtsuki3
1Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan; Radioisotope Center, The University of Tokyo, Tokyo, Japan.
Abstract:
Stress induces various responses, including translational suppression and tRNA degradation in mammals. Previously, we showed that heat stress induces degradation of initiator tRNA(Met) (iMet) through 5'-3' exoribonuclease Xrn1 and Xrn2, respectively. In addition, we found that rapamycin inhibits the degradation of iMet under heat stress conditions. Here, we report that the mammalian target of rapamycin (mTOR) regulates the diffusion of Xrn2 from the nucleolus to the nucleoplasm, facilitating the degradation of iMet under conditions of heat stress. Our results suggest a mechanism of translational suppression through mTOR-regulated iMet degradation in mammalian cells.
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