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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Related Experiment Video

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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
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Development and validation of a UHPLC diode array detector method for meropenem quantification in human plasma.

Gregori Casals1, Cristina Hernández2, Susana Hidalgo1

  • 1Pharmacology and Toxicology Laboratory, Biochemistry and Molecular Genetics, Centro de Diagnóstico Biomédico, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), IDIBAPS, Hospital Clínic de Barcelona, Barcelona University, C/Villarroel 170, 08036 Barcelona, Spain.

Clinical Biochemistry
|August 17, 2014
PubMed
Summary

A new ultra-high performance liquid chromatography method accurately quantifies meropenem in plasma. Many critically ill patients had sub-therapeutic meropenem levels, indicating a need for therapeutic drug monitoring.

Keywords:
ClearanceMeropenemPharmacokineticsTherapeutic drug monitoringUHPLC

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Critical Care Medicine

Background:

  • Meropenem is a vital β-lactam antibiotic for severe infections in intensive care units.
  • Accurate quantification of meropenem is crucial for effective treatment and patient outcomes.

Purpose of the Study:

  • To develop and validate a sensitive and specific ultra-high performance liquid chromatography (UHPLC) method for meropenem quantification in human plasma.
  • To assess the method's applicability for therapeutic drug monitoring in critically ill patients.

Main Methods:

  • Method validation followed FDA guidelines for bioanalytical methods.
  • UHPLC with photodiode array detection was employed.
  • Meropenem concentrations were measured in plasma samples from 10 critically ill patients at 0, 2, 4, and 8 hours post-infusion.

Main Results:

  • The UHPLC method demonstrated high sensitivity and specificity, with quantification limits of 0.5 μg/mL.
  • Intraday and day-to-day variations were below 10%, with accuracy between 94% and 114%.
  • Significant interindividual pharmacokinetic variability was observed; 50% of patients had sub-therapeutic meropenem levels at 8 hours, linked to high creatinine clearance.

Conclusions:

  • The validated UHPLC method is suitable for pharmacokinetic studies and routine clinical monitoring of meropenem.
  • Therapeutic drug monitoring of meropenem is recommended for critically ill patients to ensure efficacy.