Multiplexed aberration measurement for deep tissue imaging in vivo
Chen Wang1, Rui Liu2, Daniel E Milkie3
11] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2] State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China. [3].
Abstract:
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
