Related Experiment Video
Updated: Apr 25, 2026
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
Published on: July 17, 2016
[Protective effect of bone marrow mesenchymal stem cell-derived microvesicles on glutamate injured PC12 cells]
Shan-Shan Lin1, Bo Zhu2, Zi-Kuan Guo3
1Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
Abstract:
This study was aimed to investigate the protective effect of bone mesenchymal stem cell-derived microvesicles (BMMSC-MV) on glutamate injured PC12 cells so as to elucidate the mechanism of the neural damage repair. BMMSC were isolated and purified with density-gradient centrifugation method, BMMSC-MV were harvested from the supernatants of BMMSC by hypothermal ultracentrifugation method. The surface markers of BMMSC reacted against different antibodies were detected by flow cytometry. The morphology features of MV were observed under an electron microscope. Experiment was divided into three groups, one was a control group, and the other two were glutamate-injured group and co-culture group of BMMSC-MV and glutamate-damaged cells respectively. MTT test was used to evaluate the proliferative status of PC12 cells and the AnnexinV-FITC detecting kit and Hoechst33342 were used to detect the apoptosis of PC12 cells in different groups. The results showed that BMMSC isolated from rat bone marrow were highly positive for CD29, CD44 and negative for CD31, CD34 and CD45. The morphology of MV was round and the vesicles were homogenous in size. BMMSC-MV exhibited a protective effect on the excitotoxicity-injured PC12 cells, displaying increase of cell viability, decrease of Annexin-V/PI staining positive and nuclear condensed cells. It is concluded that BMMSC-MV can protect PC12 cells from glutamate-induced apoptosis, suggesting that BMMSC-MV may be a potential candidate for treatment of neurological diseases.This study provides the preliminary experimental and theoretical evidence for use of BMMSC-MV in treatment of neural excited damage.
Insights
Bone mesenchymal stem cell-derived microvesicles (BMMSC-MV) protect against glutamate-induced neural damage in PC12 cells. This suggests BMMSC-MV show promise for treating neurological diseases by reducing cell apoptosis.
Area of Science:
- Neuroscience
- Stem Cell Biology
- Biotechnology
Background:
- Glutamate excitotoxicity is a significant factor in neurological damage.
- Neural damage repair mechanisms are crucial for treating neurodegenerative diseases.
- Bone mesenchymal stem cell-derived microvesicles (BMMSC-MV) are being explored for therapeutic potential.
Purpose of the Study:
- To investigate the protective effects of BMMSC-MV on glutamate-injured PC12 cells.
- To elucidate the mechanism by which BMMSC-MV facilitate neural damage repair.
- To assess the potential of BMMSC-MV as a therapeutic agent for neurological disorders.
Main Methods:
- Isolation and purification of BMMSC and harvesting of BMMSC-MV.
- Characterization of BMMSC surface markers via flow cytometry and MV morphology via electron microscopy.
- Assessment of PC12 cell viability (MTT assay) and apoptosis (Annexin V/Hoechst 33342 staining) in control, glutamate-injured, and BMMSC-MV co-cultured groups.
Main Results:
- BMMSC confirmed positive for CD29, CD44 and negative for CD31, CD34, CD45.
- BMMSC-MV displayed characteristic morphology (round, homogenous size).
- BMMSC-MV significantly increased viability and decreased apoptosis in glutamate-injured PC12 cells.
Conclusions:
- BMMSC-MV demonstrate a protective effect against glutamate-induced excitotoxicity in PC12 cells.
- BMMSC-MV reduce apoptosis, indicating a role in neural damage repair.
- BMMSC-MV represent a potential therapeutic candidate for neurological diseases.
