Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

3.4K
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An investigation of the major volatile organic compounds released by maize plants following the application of methyl jasmonate and Z-jasmone.

Journal of plant physiology·2026
Same author

Honey microbiome as a source of bioactive metabolites: the role of the microorganisms and the influence of physicochemical parameters.

Journal of chromatography. A·2026
Same author

Solid-Phase Microextraction (SPME) for Food Safety: Recent Innovations in Monitoring Anthropogenic Endocrine Disruptors.

Critical reviews in analytical chemistry·2026
Same author

Selected hazardous elements in food: Chemical analysis including speciation and biological properties.

Food chemistry·2025
Same author

Evaluation of Quality of Life in Adult Celiac Patients Living in Lithuania and Their Compliance with a Gluten-Free Diet: A Pilot Study.

Medicina (Kaunas, Lithuania)·2025
Same author

Determination of selected isoquinoline alkaloids from Corydalis species extracts by liquid chromatography and their in vitro and in silico cholinesterases inhibition and in vitro and in vivo cytotoxic activity against human melanoma cells.

Fitoterapia·2025

Related Experiment Video

Updated: Apr 25, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

7.8K

Urine sample preparation for proteomic analysis.

Pawel Olszowy1, Boguslaw Buszewski

  • 1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University, Torun, Poland.

Journal of Separation Science
|August 19, 2014
PubMed
Summary
This summary is machine-generated.

Optimizing urine sample preparation is crucial for accurate proteomic analysis and identifying cancer biomarkers. Proper methods ensure protein integrity, preventing loss of vital diagnostic information.

Keywords:
Liquid chromatographyMass spectrometryProteomicsSample preparationUrine

More Related Videos

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.4K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

793

Related Experiment Videos

Last Updated: Apr 25, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

7.8K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.4K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

793

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biomarker Discovery

Background:

  • Sample preparation is a significant bottleneck in analytical processes, especially for biological matrices.
  • Proteomic analysis is particularly sensitive to sample preparation due to potential cross-reactions and unintended protein modifications (e.g., hydrolysis, degradation).
  • Inadequate sample processing can reduce protein sequence coverage, hindering accurate identification.

Purpose of the Study:

  • To review obstacles and achievements in urine sample preparation for proteome analysis.
  • To highlight the utility of urine as a valuable biological matrix for large-scale studies.
  • To focus on applications in the discovery of novel cancer biomarkers.

Main Methods:

  • The review summarizes various methods and tools used for protein sample preparation from urine.
  • Mass spectrometry-based analytical techniques are considered for proteome analysis.
  • The focus is on evaluating protocols for their effectiveness in preserving protein integrity.

Main Results:

  • Challenges in urine sample preparation include maintaining protein integrity and avoiding artifacts.
  • Successful protocols enable comprehensive proteome analysis and identification of proteins.
  • Urine presents a promising matrix for biomarker discovery when sample preparation is optimized.

Conclusions:

  • Effective urine sample preparation is essential for reliable proteomic analysis.
  • Optimized protocols enhance the potential of urine as a source for novel cancer biomarker discovery.
  • Further research into sample preparation techniques will advance the clinical application of urine proteomics.