Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

29
Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.
29
Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

42
An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...
42
Hemorrhagic Stroke l: Introduction01:17

Hemorrhagic Stroke l: Introduction

15
A hemorrhagic stroke is an acute neurological event that occurs when a weakened cerebral blood vessel ruptures, allowing blood to accumulate within or around the brain. The sudden release of blood forms a focal hematoma that increases intracranial pressure, displaces neural tissue, and can obstruct cerebrospinal fluid pathways. These effects may be compounded by intraventricular extension of the hemorrhage, cerebral edema, or compression of adjacent structures, all of which contribute to...
15
Transient Ischemic Attack l: Introduction01:26

Transient Ischemic Attack l: Introduction

13
A transient ischemic attack (TIA) is a brief episode of neurological dysfunction caused by a temporary, focal reduction in cerebral blood flow. Although symptoms resemble those of an ischemic stroke, the interruption in perfusion is short-lived and does not cause permanent infarction. TIAs are clinically important because they often serve as early warning events for future stroke.Mechanisms of Transient Cerebral IschemiaTransient cerebral ischemia may arise through several mechanisms. One...
13
Hemorrhagic Stroke ll: Pathophysiology01:29

Hemorrhagic Stroke ll: Pathophysiology

14
A hemorrhagic stroke develops when a cerebral blood vessel ruptures, allowing blood to escape into the surrounding brain tissue, as in intracerebral hemorrhage (ICH), or into the subarachnoid space, as in subarachnoid hemorrhage (SAH). Because the skull is a rigid compartment, the sudden presence of extravascular blood rapidly increases intracranial pressure and compresses adjacent neural structures, leading to immediate tissue injury and impaired cerebral perfusion.Mass Effect and Primary...
14

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-Based Biomarkers for Diagnostic Discrimination of Ischemic and Hemorrhagic Stroke: A Systematic Review.

Journal of clinical medicine·2026
Same author

Graphic-style stories to engage limited resource communities and promote health: methods for iterative co-design with community representatives.

Frontiers in public health·2025
Same author

Hispanic and Latino perspectives on stroke and rehabilitation research in the midwestern United States: an exploratory mixed methods study.

Physiotherapy theory and practice·2025
Same author

Protocol for the HIT-Stroke Trial 2 randomized controlled trial: Moderate-intensity exercise versus high-intensity interval training to determine the optimal training intensity for walking rehabilitation in chronic stroke.

medRxiv : the preprint server for health sciences·2025
Same author

Serum NFL and neuropsychological performance over ∼8 years in women with and without HIV: a longitudinal repeated measures study.

EClinicalMedicine·2025
Same author

Blood-based protein biomarkers during the acute ischemic stroke treatment window: a systematic review.

Frontiers in neurology·2024
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

Related Experiment Video

Updated: Apr 25, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

572

Expression profile based gene clusters for ischemic stroke detection.

Mateusz G Adamski1, Yan Li2, Erin Wagner2

  • 1Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Department of Neurology, Jagiellonian University Medical College, ul. Botaniczna 3, Krakow 31-501, Poland.

Genomics
|August 20, 2014
PubMed
Summary
This summary is machine-generated.

This study identifies gene expression patterns in blood cells to improve diagnosis of ischemic stroke. Specific transcript clusters show high accuracy, offering a promising tool for detecting acute ischemic stroke.

Keywords:
BiomarkerGene expressionStrokeTranscriptomeqPCR

More Related Videos

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
14:27

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

Published on: June 26, 2013

15.2K
Simultaneous Photothrombosis and Fiber Photometry to Induce and Monitor Ischemic Stroke in Behaving Mice
07:49

Simultaneous Photothrombosis and Fiber Photometry to Induce and Monitor Ischemic Stroke in Behaving Mice

Published on: November 14, 2025

686

Related Experiment Videos

Last Updated: Apr 25, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

572
Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
14:27

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

Published on: June 26, 2013

15.2K
Simultaneous Photothrombosis and Fiber Photometry to Induce and Monitor Ischemic Stroke in Behaving Mice
07:49

Simultaneous Photothrombosis and Fiber Photometry to Induce and Monitor Ischemic Stroke in Behaving Mice

Published on: November 14, 2025

686

Area of Science:

  • Biochemistry
  • Genomics
  • Medical Diagnostics

Background:

  • Gene expression alterations in leukocytes show potential for ischemic stroke diagnosis.
  • Previous microarray studies identified candidate diagnostic markers.

Purpose of the Study:

  • Quantitate individual transcript expression of 40 candidate markers.
  • Identify transcript clusters for ischemic stroke detection.
  • Assess the clinical diagnostic utility of identified clusters.

Main Methods:

  • Utilized high-throughput, next-generation quantitative polymerase chain reaction (qPCR).
  • Analyzed gene expression profiles from circulating leukocytes.
  • Performed cluster analysis to identify discriminating transcript sets.

Main Results:

  • 16 of 40 transcripts were significantly up-regulated in stroke patients (p<0.05).
  • Six transcript clusters differentiated stroke from control subjects (p values from 1.01e-9 to 0.03).
  • A 7-transcript cluster achieved high accuracy for stroke classification (AUC=0.854).

Conclusions:

  • Validated and enhanced the diagnostic value of previously identified transcripts for ischemic stroke.
  • Identified promising gene expression clusters for acute ischemic stroke detection.