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Updated: Apr 25, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Maize transformation technology development for commercial event generation.

Qiudeng Que¹, Sivamani Elumalai¹, Xianggan Li¹

¹Syngenta Biotechnology, Inc. Research Triangle Park, NC, USA.

Frontiers in Plant Science

|August 21, 2014

Summary

This summary is machine-generated.

Maize biotechnology is advancing rapidly, with Agrobacterium-mediated transformation enabling efficient development of new biotech traits. This review covers current methods for producing transgenic maize events and future directions in gene stacking and genome modification.

Keywords:

Agrobacterium T-DNA genome modification maize transformation multi-gene stack targeted insertion trait development transgenic events

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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Published on: February 14, 2020

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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Area of Science:

Agricultural Science
Biotechnology
Plant Genetics

Background:

Maize is a globally significant food and feed crop, with extensive application of biotechnology.
More biotech traits are available in maize than any other crop, highlighting its importance in agricultural innovation.
Efficient generation of high-quality transgenic events is crucial for commercializing biotech traits in maize.

Purpose of the Study:

To review advancements in Agrobacterium-mediated maize transformation technologies.
To discuss current transformation systems for commercial maize traits.
To explore strategies for efficient transformation of multi-gene vectors and genome modification.

Main Methods:

Review of published literature on maize transformation techniques.
Analysis of Agrobacterium-mediated transformation protocols.
Discussion of vector assembly and gene stacking strategies.
Examination of site-directed nuclease technologies for genome editing.

Main Results:

Significant progress has been made in Agrobacterium-mediated maize transformation since 1996.
Various transformation systems are employed for commercial transgenic event production.
New tools are needed for efficient transformation with multiple trait genes.
Site-directed nucleases offer potential for targeted genome modification.

Conclusions:

Agrobacterium-mediated transformation is a key technology for maize trait development.
Efficient high-throughput systems are essential for commercial applications.
Future research focuses on gene stacking and targeted genome modification for enhanced crop traits.