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Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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Visualizing life with ambient mass spectrometry.

Cheng-Chih Hsu1, Pieter C Dorrestein2

  • 1Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, United States.

Current Opinion in Biotechnology
|August 23, 2014
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Summary
This summary is machine-generated.

Ambient ionization mass spectrometry, including desorption electrospray ionization (DESI), offers versatile applications in life sciences. Recent technological advances and multimodal integration are expanding its use in biology and medicine.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Life Sciences

Background:

  • Desorption electrospray ionization (DESI) has spurred the development of numerous ambient ionization techniques.
  • Ambient and atmospheric pressure mass spectrometry are increasingly utilized across diverse biological fields.

Purpose of the Study:

  • To review key technological advancements in ambient mass spectrometry.
  • To highlight the expanding applications of these techniques in life sciences.

Main Methods:

  • Review of influential technological advances in ambient mass spectrometry.
  • Discussion of multimodal integration with other biotechnologies.
  • Exploration of computational methods for data processing.

Main Results:

  • Ambient ionization mass spectrometry is applied in plant science, microbiology, neuroscience, and cancer pathology.
  • Integration with other biotechnologies and advanced computational methods are emerging areas.
  • Technological progress continues to broaden the scope of biological analyses.

Conclusions:

  • Ambient mass spectrometry represents a rapidly evolving field with significant impact on life science research.
  • Future directions involve multimodal integration and sophisticated data analysis.
  • The technology facilitates diverse biological applications from basic research to pathology.