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Related Experiment Video

Updated: Apr 25, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

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Profiling of embryonic stem cell differentiation.

Nobuaki Shiraki1, Soichiro Ogaki1, Shoen Kume1

  • 1Department of Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2-2-1, Kumamoto 860-0811, Japan.

The Review of Diabetic Studies : RDS
|August 23, 2014
PubMed
Summary
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Embryonic stem cells aid developmental biology research. Profiling studies identified novel genes and pathways in mouse embryos and human pancreatic cells, revealing new markers for endoderm and pancreas development.

Area of Science:

  • Developmental Biology
  • Stem Cell Research
  • Genomics

Background:

  • Embryonic stem (ES) cells mimic normal developmental stages, making them valuable tools for studying developmental biology.
  • Profiling of ES cell-derived cells provides insights into differentiated cell characteristics and aids in identifying novel differentiation markers and pathways.

Purpose of the Study:

  • To review recent profiling studies of mouse embryos, human islets, and human ES cell-derived differentiated cells.
  • To identify novel endoderm and pancreatic progenitor marker genes using gene expression profiling.
  • To discuss the benefits of using ES cells for studying early embryonic development.

Main Methods:

  • Analysis of global gene expression data from mouse embryos.
  • Examination of gene expression databases for human pancreas cells (Beta Cell Gene Atlas, EuroDia database).

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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
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  • Differentiation of mouse ES cells into various lineages (mesendoderm, definitive endoderm, mesoderm, ectoderm, Pdx1-expressing pancreatic) followed by DNA microarray analysis.
  • In situ hybridization to study gene expression patterns in normal embryonic development.

    • Main Results:

    • Global gene expression data from mouse embryos identified novel genes and pathways involved in development and direct reprogramming.
    • Profiling studies of human ES cell-derived pancreatic cells focused on gene expression, microRNAs, epigenetics, and protein expression.
    • Novel genes (Akr1c19, Aebp2, Pbxip1, Creb3l1) were identified as potentially novel markers for definitive endoderm and pancreatic lineages, with 27 genes expressed in definitive endoderm and 15 in pancreatic buds of mouse embryos.

    Conclusions:

    • Profiling of ES cell-derived cells offers significant benefits for understanding early embryonic development.
    • The study identified novel genes expressed in definitive endoderm and pancreatic progenitor cells, advancing the search for differentiation markers.
    • ES cells serve as a powerful model system for recapitulating developmental processes and discovering new molecular players in embryogenesis.