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Related Experiment Video

Updated: Apr 25, 2026

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

Published on: April 27, 2021

Mast cell purification protocols.

Jasmine Farrington¹, Elizabeth P Seward, Peter T Peachell

¹Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.

Methods in Molecular Biology (Clifton, N.J.)

|August 24, 2014

Summary

This summary is machine-generated.

This study details methods for isolating human lung mast cells. These techniques are adaptable for mast cell isolation from various tissues, acknowledging cellular heterogeneity.

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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

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Isolation and Activation of Murine Lymphocytes

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Published on: October 30, 2016

Area of Science:

Immunology
Cell Biology

Background:

Mast cells exhibit significant heterogeneity depending on their tissue of origin.
Established methods for mast cell isolation exist but require ongoing refinement.

Purpose of the Study:

To provide detailed protocols for the isolation and purification of human lung mast cells.
To highlight the general applicability of these methods for mast cell isolation from diverse tissues.

Main Methods:

Detailed description of established and refined techniques for mast cell isolation.
Focus on methods applicable to human lung tissue.
Discussion of broader tissue applicability.

Main Results:

Successful isolation and purification of human lung mast cells.
Demonstration of method adaptability for various tissue sources.

Conclusions:

The presented methods enable effective isolation of human lung mast cells.
These protocols are valuable for studying tissue-specific mast cell heterogeneity.