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Related Concept Videos

Disorders of Leukocytes01:27

Disorders of Leukocytes

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Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...
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Classification of Leukocytes01:30

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Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
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Related Experiment Video

Updated: Apr 25, 2026

Basophil Activation Test for Allergy Diagnosis
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The absolute basophil count.

Elena Borzova1, Clemens A Dahinden

  • 1BMRC, University of East Anglia, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 24, 2014
PubMed
Summary

Accurate absolute basophil counts are crucial. Fluorescence flow cytometry offers precise enumeration of circulating basophils, presenting a promising candidate reference method for peripheral blood analysis.

Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Accurate absolute basophil counts are essential for clinical diagnostics.
  • Manual blood smear counting, the current reference method, has recognized limitations including observer and statistical errors.
  • Automated analyzers provide high throughput but exhibit inconsistent analytical performance for basophil enumeration.

Purpose of the Study:

  • To review and describe various techniques for determining absolute basophil counts in peripheral blood.
  • To highlight the potential of fluorescence flow cytometry as a novel reference method.
  • To discuss emerging technologies for cell counting.

Main Methods:

  • Manual counting of peripheral blood smears and cell-counting chambers.

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  • Automated hematology analyzers.
  • Fluorescence flow cytometry methodologies (double-platform, bead-based, volumetric).
  • Mention of ImageStream technology and on-chip blood counting.

    • Main Results:

    • Manual counting is the reference method but has limitations.
    • Automated analyzers offer speed but lack consistent accuracy for basophils.
    • Flow cytometry demonstrates high precision and statistical superiority for basophil enumeration.
    • Analytical performance of emerging technologies for absolute basophil counts is yet to be established.

    Conclusions:

    • Fluorescence flow cytometry is a highly precise and statistically superior candidate reference method for absolute basophil counts.
    • Further validation is needed for emerging technologies like ImageStream and on-chip counting.
    • Accurate basophil enumeration is critical for reliable clinical interpretation.