Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.0K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.0K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.4K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.4K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

1.9K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
1.9K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effects of Pistacia atlantica seed-canola oil blend on serum oxidative stress and lipid biomarkers in adults with metabolic syndrome.

Scientific reports·2026
Same author

Comparative effects of pistacia Atlantica seed-canola oil and corn-canola oils with and without TBHQ on liver and kidney function tests in patients with metabolic syndrome: A triple-blind crossover clinical trial.

Journal of diabetes and metabolic disorders·2025
Same author

Epigenetic isoforms (DNMT3A/3B and HDAC3/7) potential predictors of endothelial dysfunction and subclinical atherosclerosis.

Molecular biology reports·2025
Same author

Alteration in epigenetic profile in subclinical atherosclerosis and in high uric acid.

Scientific reports·2025
Same author

Sperm DNA damage and disturbed chromatin condensation indexes (DFI and CMA3) in normozoospermic men with unexplained infertility problem.

The aging male : the official journal of the International Society for the Study of the Aging Male·2025
Same author

Liposome-Mediated MicroRNA Delivery: An Additional Layer of Gene Network Regulation and Nuclear Reprogramming

Iranian biomedical journal·2025
Same journal

Circulating miRNA in metabolic syndrome and cancer: Machinery, diagnostics, and therapeutics.

The international journal of biochemistry & cell biology·2026
Same journal

Corrigendum to "Sodium butyrate down-regulation of indoleamine 2, 3-dioxygenase at the transcriptional and post-transcriptional levels" [Int. J. Biochem. Cell Biol. 42 (2010) 1840-1846].

The international journal of biochemistry & cell biology·2026
Same journal

Whole-brain spatial metabolomics reveals metabolic gradient shifts in a murine glioma model following boron neutron capture therapy (130 characters).

The international journal of biochemistry & cell biology·2026
Same journal

LCN2 modulates Th17/Treg balance in vitro and is associated with an adaptive response to intestinal ischemia-reperfusion injury under Hmox1-deficient conditions.

The international journal of biochemistry & cell biology·2026
Same journal

Chloroquine modulates the redox-sensitive signalling via inhibiting the AMPK-ULK1 under LPS induced state in murine splenic macrophages.

The international journal of biochemistry & cell biology·2026
Same journal

The vicious cycle of hyperglycemia and oxidative stress: Novel mechanistic insights into a pathogenic alliance.

The international journal of biochemistry & cell biology·2026
See all related articles

Related Experiment Video

Updated: Apr 25, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

11.7K

MicroRNA-based system in stem cell reprogramming; differentiation/dedifferentiation.

Fatemeh Pourrajab1, Mojtaba Babaei Zarch2, Mohammad BaghiYazdi2

  • 1School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Department of Clinical Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

The International Journal of Biochemistry & Cell Biology
|August 25, 2014
PubMed
Summary
This summary is machine-generated.

MicroRNAs are key regulators of stem cell (SC) pluripotency and self-renewal. Understanding microRNA roles is vital for advancing regenerative medicine and cell reprogramming strategies.

Keywords:
Cell reprogramingMicroRNASTEM cell

More Related Videos

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.5K
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

15.4K

Related Experiment Videos

Last Updated: Apr 25, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

11.7K
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.5K
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

15.4K

Area of Science:

  • Stem cell biology
  • Molecular genetics
  • Epigenetics

Background:

  • Stem cells possess self-renewal and differentiation capabilities, crucial for development and regenerative medicine.
  • MicroRNAs (miRNAs) are critical regulators of gene expression, influencing cellular processes.
  • miRNAs are implicated in maintaining stem cell pluripotency and self-renewal.

Purpose of the Study:

  • To review the current understanding of microRNA-dependent mechanisms in maintaining stem cell pluripotency.
  • To highlight the significance of microRNA signatures in stem cell regulation.
  • To explore the potential of microRNAs in regenerative medicine applications.

Main Methods:

  • Literature review of studies on microRNAs and stem cells.
  • Analysis of molecular mechanisms governing stem cell pluripotency.
  • Integration of findings on microRNA-cell signaling interactions.

Main Results:

  • MicroRNAs play a significant role in maintaining stem cell pluripotency and self-renewal.
  • Specific microRNA signatures are associated with pluripotency maintenance.
  • Interactions between microRNAs and cell signaling pathways are critical for stem cell fate.

Conclusions:

  • MicroRNA-mediated regulation is fundamental to stem cell pluripotency.
  • Targeting microRNA pathways offers potential for cell reprogramming and directed differentiation.
  • Further research into microRNA networks will advance regenerative medicine.