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Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

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Related Experiment Video

Updated: Dec 23, 2025

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

Advances in yeast genome engineering.

Florian David¹, Verena Siewers²

¹Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden.

FEMS Yeast Research

|August 27, 2014

Summary

This summary is machine-generated.

Yeast genome engineering advances enable faster, multiple gene edits for metabolic engineering and synthetic biology. New techniques and endonucleases improve genome redesign and evolution capabilities.

Keywords:

Genome editing Sc2.0 adaptive laboratory evolution endonucleases

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Area of Science:

Microbiology and Biotechnology
Molecular Biology and Genetics

Background:

Homologous recombination has long been used for yeast genome engineering.
Yeast is increasingly vital as a cell factory and synthetic biology chassis.
Efficient introduction of multiple genetic modifications is crucial for these applications.

Purpose of the Study:

To review recent advancements in yeast genome engineering.
To highlight novel techniques for genome redesign and evolution.
To discuss the role of endonucleases in genome engineering.

Main Methods:

Review of existing and novel genome engineering techniques.
Focus on methods for introducing multiple targeted genetic changes.
Discussion of endonuclease tools for genome manipulation.

Main Results:

Improvements in existing genome engineering methods have been achieved.
Novel techniques for advanced genome redesign and evolution are emerging.
Endonucleases are important tools for efficient genome engineering.

Conclusions:

Advanced genome engineering methods are essential for yeast cell factories and synthetic biology.
Novel techniques and endonuclease tools enhance the speed and efficiency of yeast genome modification.
Continued development in this field will drive innovation in biotechnology.