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Updated: Apr 25, 2026
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
Published on: September 2, 2015
Surface presentation of functional peptides in solution determines cell internalization efficiency of TAT conjugated
Nevena Todorova1, Ciro Chiappini, Morgan Mager
1Health Innovations Research Institute, RMIT University , GPO Box 2476, Melbourne, VIC Australia.
None:
Functionalizing nanoparticles with cell-penetrating peptides is a popular choice for cellular delivery. We investigated the effects of TAT peptide concentration and arrangement in solution on functionalized nanoparticles' efficacy for membrane permeation. We found that cell internalization correlates with the positive charge distribution achieved prior to nanoparticle encountering interactions with membrane. We identified a combination of solution based properties required to maximize the internalization efficacy of TAT-functionalized nanoparticles.
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