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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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Upstream Processing01:27

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Perfusion microbioreactor for CAR-Treg manufacturing.

William Edwards1, Ningjia Sun1, Yikai Wang1,2

  • 1Centre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.

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|March 30, 2026
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Summary
This summary is machine-generated.

A novel KCL-Microbioreactor (K-MBR) platform addresses cell and gene therapy manufacturing challenges. This microfluidic device enhances therapeutic cell expansion and gene delivery, improving yield for personalized treatments.

Keywords:
Biological sciences

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Area of Science:

  • Biotechnology
  • Cell Therapy Manufacturing
  • Microfluidics

Background:

  • Cell and gene therapies (CGTs) face manufacturing hurdles in cost, consistency, and personalization.
  • Traditional bioreactors are ill-suited for decentralized, small-batch production of personalized therapies like CAR T-cells.

Purpose of the Study:

  • To develop a novel microbioreactor platform for efficient and scalable CGT manufacturing.
  • To overcome the limitations of traditional bioreactors for personalized cell therapies.

Main Methods:

  • Development of the KCL-Microbioreactor (K-MBR), a closed, microfluidic platform using polydimethylsiloxane (PDMS).
  • Integration of spatial confinement, semi-continuous perfusion, and viral transduction within the K-MBR.
  • Demonstration using CAR-Tregs targeting HLA-A2.

Main Results:

  • The K-MBR platform enables efficient gene delivery and robust expansion of therapeutic cells.
  • Functional CAR-Tregs targeting HLA-A2 were successfully generated.
  • Achieved a 92% increase in yield compared to conventional manufacturing methods.

Conclusions:

  • The K-MBR offers a streamlined solution for CGT manufacturing, enhancing scalability.
  • This platform has the potential to reduce production costs for a wide range of cell therapies.
  • The K-MBR facilitates efficient production of personalized cell therapies.